Patrick Thatayaone Mokgethi
Laboratory Scientist / Research Student
GABORONE
Summary
A conscientious and dedicated professional with extensive experience in laboratory and research fields. A highly organized and efficient individual who can prioritize his work to ensure deadlines are met while maintaining a high standard of analysis and insight. This high-performing individual can work well under pressure, consistently delivering excellent, quick, accurate results. I am self-motivated and thus work very well independently but I am just as resourceful working in a team.
Overview
10
10
years of professional experience
6
6
years of post-secondary education
1
1
Certification
Work History
Laboratory Scientist II
Botswana Harvard AIDS Institute Partnership
12.2016 - Current
- COVID-19 Laboratory scientists specifically carrying out RNA Extraction and Detection
- I am the principal investigator in the project Virologic and Molecular Characterisation of HIV-1 in Non-Citizens in Botswana (PhD)
- Phodiso Study Laboratory Coordinator (2023-current)
- I was also involved in an investigation of Undetectable HIV DNA in Perinatally Infected Adolescents on Long-term ART wherein I learned and developed the skill of harvesting and isolating Peripheral blood mononuclear cells
- I was a Laboratory Scientist in the Project; Botswana Combination Prevention Project (BCPP), a PEPFAR-funded community randomized trial designed to evaluate the impact of a combination prevention package on HIV incidence
- Other Responsibilities include;
- Training and supervision of new laboratory staff, students, and fellows
- Operating requisite Laboratory equipment and instruments; recording data and maintaining lab equipment
- Extraction of Nucleic Acids (DNA, RNA) from various samples (blood, plasma), Run DNA Polymerase Chain Reaction (PCR) from extracted RNA, DNA
- Run Gel Electrophoresis from amplified DNA
- Sequencing and analysis of amplified DNA
- Preparation of sterile media such as broth, and agar in plates, jars, or test tubes in growing microbial culture
- Preparation of solutions and reagents following standard laboratory procedures and formulas
- Troubleshooting Laboratory procedures to ensure quality performance and improvement
- Assisting with the editing of Manuscripts, grants, scientific articles, and presentations as required
Laboratory Consultant
B-TECH
01.2020 - 01.2022
- Providing Technical Assistance and Direct service delivery
- Human Papillomavirus DNA testing
Laboratory Assistant and Demonstrator
University of Botswana
10.2014 - 11.2015
- Company Overview: Department of Environmental Health
- Preparations for lab practical sessions
- Lab demonstrations to students during practical sessions
- Sorting of calibration of department equipment
- Assisted in drawing up a departmental inventory
- Issuing of equipment from the lab to students and staff
- Department of Environmental Health
Education
Bachelor of Science - Biological Sciences
University of Botswana
Gaborone, Botswana 08.2009 - 05.2014
BGCSE - undefined
Madiba Senior Secondary School
01.2007 - 01.2008
Skills
DNA/RNA Extraction
Automated Extraction Platforms
Manual Extraction
QIAsymphony
QIAGEN Easy 1
Nuclisens EasyMag
Abbott sp2000
MGISP-960
DNA/RNA polymerase Chain Reaction (PCR)
Gel Electrophoresis
Sequencing
Next Generation Sequencing
Sanger Sequencing
Peripheral Blood Mononuclear Cell Isolation
Sample Reception
DNA PCR
Viral Load Quantification
CD4 count
Serology
Chemistry
Hematology
Scientific writing
Research design
Data management
Publications
- High prevalence of pre-treatment and acquired HIV-1 drug resistance mutations among non-citizens living with HIV in Botswana., Mokgethi PT, Choga WT, Maruapula D, Moraka NO, Seatla KK, Bareng OT, Ditshwanelo DD, Mulenga G, Mohammed T, Kaumba PM, Chihungwa M, Marukutira T, Moyo S, Kofhethile CK, Dickinson D, Mpoloka SW and Gaseitsiwe S, Front. Microbiol., 15, 1338191, 10.3389/fmicb.2024.1338191, 2024
- Archived rilpivirine-associated resistance mutations among ART-naive and virologically suppressed people living with HIV-1 subtype C in Botswana: implications for cabotegravir/rilpivirine use., Dorcas Maruapula, Natasha O Moraka, Ontlametse T Bareng, Patrick T Mokgethi, Wonderful T Choga, Kaelo K Seatla, Nametso Kelentse, Catherine K Koofhethille, Boitumelo J L Zuze, Tendani Gaolathe, Molly Pretorius-Holme, Joseph Makhema, Vlad Novitsky, Roger Shapiro, Sikhulile Moyo, Shahin Lockman, Simani Gaseitsiwe, Journal of Antimicrobial Chemotherapy, 78, 10, 2489–2495, 2023, 10.1093/jac/dkad258
- Low rates of nucleoside reverse transcriptase inhibitor and nonnucleoside reverse transcriptase inhibitor drug resistance in Botswana., Moyo S, Gaseitsiwe S, Zahralban-Steele M, Maruapula D, Nkhisang T, Mokaleng B, Mohammed T, Ditlhako TR, Bareng OT, Mokgethi TP, van Widenfelt E, Pretorius-Holme M, Mine MO, Raizes E, Yankinda EK, Wirth KE, Gaolathe T, Makhema JM, Lockman S, Essex M, Novitsky V., AIDS, 2019-05-01, 33, 6, 1073-1082, 10.1097/QAD.0000000000002166
References
- Dr. Simani Gaseitsiwe, Research Associate, Harvard T. H. Chan School of Public Health and Botswana Harvard AIDS Institute Partnership, sgaseitsiwe@bhp.org.bw, (+267) 390 2671
- Dr. Catherine K Koofhethile, Research Associate, Harvard T. H. Chan School of Public Health and Botswana Harvard AIDS Institute Partnership, kckoofhethile@hsph.harvard.edu, (+267) 390 2671
- Dr Tafi Marukutira, Senior Research Fellow at Burnet Institute, Adjunct Research Fellow at Monash University and Research Associate at Botswana Harvard Health Partnership, tafireyi.marukutira@burnet.edu.au
- Prof. Sikhulile Moyo, Research Associate, Harvard T. H. Chan School of Public Health and Laboratory Director at Botswana Harvard AIDS Institute Partnership, smoyo@bhp.org.bw, (+267) 390 2671
- Prof Bontle Mbongwe, Senior Lecturer at University of Botswana Toxicology, Public Health, Environmental Health, Bontle.mbongwe@gmail.com, (+267) 355 5238
Training
- Harvard Biostatistics Course for Sub-Saharan African Students, 2023
- Harvard R and Biostatistics Course, 2023
- IBT Bioinformatics Courses (H3Bionet), 2023
- Wellcome Connecting Science Course: Next Generation Sequencing, 03/24/23 - 03/31/23, Wellcome Genome Campus, Hinxton, Cambridge, UK
Personal Information
- Hobbies: Reading, Basketball, Dog Grooming
- Date of Birth: 02/02/91
Certification
Certified HIV Genotypic Resistance Testing
Timeline
Laboratory Consultant
B-TECH
01.2020 - 01.2022
Laboratory Scientist II
Botswana Harvard AIDS Institute Partnership
12.2016 - Current
Laboratory Assistant and Demonstrator
University of Botswana
10.2014 - 11.2015
Bachelor of Science - Biological Sciences
University of Botswana
08.2009 - 05.2014
BGCSE - undefined
Madiba Senior Secondary School
01.2007 - 01.2008