A conscientious and dedicated professional with extensive experience in laboratory and research fields. A highly organized and efficient individual who can prioritize his work to ensure deadlines are met while maintaining a high standard of analysis and insight. This high-performing individual can work well under pressure, consistently delivering excellent, quick, accurate results. I am self-motivated and thus work very well independently but I am just as resourceful working in a team.
Overview
10
10
years of professional experience
6
6
years of post-secondary education
1
1
Certification
Work History
Laboratory Scientist II
Botswana Harvard AIDS Institute Partnership
12.2016 - Current
COVID-19 Laboratory scientists specifically carrying out RNA Extraction and Detection
I am the principal investigator in the project Virologic and Molecular Characterisation of HIV-1 in Non-Citizens in Botswana (PhD)
Phodiso Study Laboratory Coordinator (2023-current)
I was also involved in an investigation of Undetectable HIV DNA in Perinatally Infected Adolescents on Long-term ART wherein I learned and developed the skill of harvesting and isolating Peripheral blood mononuclear cells
I was a Laboratory Scientist in the Project; Botswana Combination Prevention Project (BCPP), a PEPFAR-funded community randomized trial designed to evaluate the impact of a combination prevention package on HIV incidence
Other Responsibilities include;
Training and supervision of new laboratory staff, students, and fellows
Operating requisite Laboratory equipment and instruments; recording data and maintaining lab equipment
Extraction of Nucleic Acids (DNA, RNA) from various samples (blood, plasma), Run DNA Polymerase Chain Reaction (PCR) from extracted RNA, DNA
Run Gel Electrophoresis from amplified DNA
Sequencing and analysis of amplified DNA
Preparation of sterile media such as broth, and agar in plates, jars, or test tubes in growing microbial culture
Preparation of solutions and reagents following standard laboratory procedures and formulas
Troubleshooting Laboratory procedures to ensure quality performance and improvement
Assisting with the editing of Manuscripts, grants, scientific articles, and presentations as required
Laboratory Consultant
B-TECH
01.2020 - 01.2022
Providing Technical Assistance and Direct service delivery
Human Papillomavirus DNA testing
Laboratory Assistant and Demonstrator
University of Botswana
10.2014 - 11.2015
Company Overview: Department of Environmental Health
Preparations for lab practical sessions
Lab demonstrations to students during practical sessions
Sorting of calibration of department equipment
Assisted in drawing up a departmental inventory
Issuing of equipment from the lab to students and staff
Department of Environmental Health
Education
Bachelor of Science - Biological Sciences
University of Botswana
Gaborone, Botswana
08.2009 - 05.2014
BGCSE - undefined
Madiba Senior Secondary School
01.2007 - 01.2008
Skills
DNA/RNA Extraction
Publications
High prevalence of pre-treatment and acquired HIV-1 drug resistance mutations among non-citizens living with HIV in Botswana., Mokgethi PT, Choga WT, Maruapula D, Moraka NO, Seatla KK, Bareng OT, Ditshwanelo DD, Mulenga G, Mohammed T, Kaumba PM, Chihungwa M, Marukutira T, Moyo S, Kofhethile CK, Dickinson D, Mpoloka SW and Gaseitsiwe S, Front. Microbiol., 15, 1338191, 10.3389/fmicb.2024.1338191, 2024
Archived rilpivirine-associated resistance mutations among ART-naive and virologically suppressed people living with HIV-1 subtype C in Botswana: implications for cabotegravir/rilpivirine use., Dorcas Maruapula, Natasha O Moraka, Ontlametse T Bareng, Patrick T Mokgethi, Wonderful T Choga, Kaelo K Seatla, Nametso Kelentse, Catherine K Koofhethille, Boitumelo J L Zuze, Tendani Gaolathe, Molly Pretorius-Holme, Joseph Makhema, Vlad Novitsky, Roger Shapiro, Sikhulile Moyo, Shahin Lockman, Simani Gaseitsiwe, Journal of Antimicrobial Chemotherapy, 78, 10, 2489–2495, 2023, 10.1093/jac/dkad258
Low rates of nucleoside reverse transcriptase inhibitor and nonnucleoside reverse transcriptase inhibitor drug resistance in Botswana., Moyo S, Gaseitsiwe S, Zahralban-Steele M, Maruapula D, Nkhisang T, Mokaleng B, Mohammed T, Ditlhako TR, Bareng OT, Mokgethi TP, van Widenfelt E, Pretorius-Holme M, Mine MO, Raizes E, Yankinda EK, Wirth KE, Gaolathe T, Makhema JM, Lockman S, Essex M, Novitsky V., AIDS, 2019-05-01, 33, 6, 1073-1082, 10.1097/QAD.0000000000002166
References
Dr. Simani Gaseitsiwe, Research Associate, Harvard T. H. Chan School of Public Health and Botswana Harvard AIDS Institute Partnership, sgaseitsiwe@bhp.org.bw, (+267) 390 2671
Dr. Catherine K Koofhethile, Research Associate, Harvard T. H. Chan School of Public Health and Botswana Harvard AIDS Institute Partnership, kckoofhethile@hsph.harvard.edu, (+267) 390 2671
Dr Tafi Marukutira, Senior Research Fellow at Burnet Institute, Adjunct Research Fellow at Monash University and Research Associate at Botswana Harvard Health Partnership, tafireyi.marukutira@burnet.edu.au
Prof. Sikhulile Moyo, Research Associate, Harvard T. H. Chan School of Public Health and Laboratory Director at Botswana Harvard AIDS Institute Partnership, smoyo@bhp.org.bw, (+267) 390 2671
Prof Bontle Mbongwe, Senior Lecturer at University of Botswana Toxicology, Public Health, Environmental Health, Bontle.mbongwe@gmail.com, (+267) 355 5238
Training
Harvard Biostatistics Course for Sub-Saharan African Students, 2023
Harvard R and Biostatistics Course, 2023
IBT Bioinformatics Courses (H3Bionet), 2023
Wellcome Connecting Science Course: Next Generation Sequencing, 03/24/23 - 03/31/23, Wellcome Genome Campus, Hinxton, Cambridge, UK
Graduate Research Assistant at University of Botswana (Cell Culture Laboratory)Graduate Research Assistant at University of Botswana (Cell Culture Laboratory)