Molecular and cell biologist with post PhD experience in academic and industrial setting understanding cancer, immunology and developmental biology in vitro and in vivo. Expertise in wide array of molecular and analytical techniques. Skilled in experiment design, conduct experiments, troubleshooting, data analysis, writing and communication. Knowledge of ICH- GCP guidelines.
• Literature search, study design, conduct experiments, data analysis, data presentation in
lab & conference, writing and publication.
• Manage project & manage teams (5-10 people), establish & coordinate with collaborators.
• Data sharing within and outside lab.
• Writing and follow SOP.
• Managing transgenic mouse line, genotyping, breeding.
• Managing lab stocks of reagents and labware.
• Review publications and projects.
• Parameters optimization for isolation of mono- and multi- nucleated cells using flow
cytometry (FACS) based approach.
• Single cell RNA sequencing of cells isolated after FACS.
• Identified a novel regulator of osteoclastogenesis.
• Isolation of bone marrow and spleenocytes from mice.
• Design the prototype for DNA extraction kit from human sputum samples for in vitro
diagnostics (IVD).
• Design, optimize and validate the probes for RT-PCR based detection of M. tuberculosis
in human sputum samples for in vitro diagnostics (IVD).
• Training and supervise junior staff.
• Ordering and managing reagents.
• Setup lab, writing and follow SOP.
TECHNICAL SKILLS: Cell culture, cell based assays, flow cytometry, fluorescent microscopy imaging, immunological techniques (ELISA), immunohistochemistry (IHC), immunofluorescence, animal handling, bioinformatics (single cell RNAseq analysis, data mining), molecular biology (cloning, RNA extraction, DNA extraction, SDS-PAGE, western blotting), microbiology
undefinedi. Anuj A, Reuven N, Elson A, Stefen GE Roberts. BASP1 down-regulates RANKL induced osteocalstogenesis. Exp. Cell Res. (2023). (doi: 10.1016/j.yexcr.2023.113758).
ii. Elson A, Anuj A, Barnea-Zohar M, Reuven N. The origins and formation of bone- resorbing osteoclasts. Bone (2022) (doi: 10.1016/j.bone.2022.116538).
iii. Anuj, Lakshmi A, Ganesh V and Rayala S.K. Increased expression of miR-551a by c-Fos reduces Focal Adhesion Kinase (FAK) levels and blocks tumorigenesis. Mol Cell Biol (2019) (doi: 10.1128/MCB.00577-18).
iv. Anuj, Lakshmi A., Surabhi R.P., Kanakrajan A., Sundaram S., Pitani R. S., Mudduva L., Kremerskothen J., Venkatraman G., Rayala S.K. KIBRA attains oncogenic activity by repressing RASSF1A. British Journal of Cancer (2017) (doi:10.1038/bjc.2017.192).
v. Poster presentation at 10thFISEB/ILANIT conference held at Eilat, Israel February 2023.
vi. 8th International Mutant p53 workshop and p53 isoform, held at Lyon, May 2019.
vii. EACR-AACR-ISCR Conference: The cutting edge of contemporary cancer research, Jerusalem, Israel, October 2018.
viii. Poster presentation at EACR- 24thBiennial Congress of the European Association for Cancer Research, Manchester, UK. Anuj, Venkatraman G., Rayala S.K (2016).
i. Fellowship for pursuing Ph. D. by MHRD, Government of India with qualifying CSIR-NET JRF exam (June 2010, All India Rank-93)
ii. Fellowship for pursuing M. Tech by Department of Biotechnology, Government of India with qualifying Combined Entrance Exam for Biotechnology (CEEB), 2008 (All India Rank-7)
iii. Reviewer for journals Aging, Oncotarget
iv. Member (Ambassador) EACROctober 2018- October 2021
v. Treasurer and organizing member for BIOMERS 2013, a student symposium at Department of Biotechnology, IIT Madras, Chennai, India
vi. Volunteered for Non-Government Organization (NGO) Bhumi (Chennai), India
vii. Voluntary participation in Adult Literacy Program for Migratory Population at Regional Resource Centre, Panjab University, Chandigarh, India (2007)Participated in Adult Literacy Program for Migratory Population at Regional Resource Centre, Panjab University, Chandigarh, India (2007).